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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   BLNK Antibody   

BLNK Antibody

Purified Mouse Monoclonal Antibody

  • E - BLNK Antibody AO1582a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - BLNK Antibody AO1582a
    Figure 1: Western blot analysis using BLNK mAb against human BLNK (AA: 34-216) recombinant protein. (Expected MW is 60 kDa)
  • WB - BLNK Antibody AO1582a
    Figure 2: Western blot analysis using BLNK mouse mAb against NIH/3T3 (1) and BCBL-1 (2) cell lysate.
  • IHC - BLNK Antibody AO1582a
    Figure 3: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using BLNK mouse mAb with DAB staining.
  • IF - BLNK Antibody AO1582a
    Figure 4: Immunofluorescence analysis of HepG2 cells using BLNK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - BLNK Antibody AO1582a
    Figure 5: Flow cytometric analysis of NIH/3T3 cells using BLNK mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8WV28
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 5G9
Isotype IgG1
Calculated MW 68kDa
Description This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human BLNK expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 29760
Other Names B-cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, SLP-65, BLNK, BASH, SLP65
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBLNK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms BASH, SLP65
Function Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR- mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.
Cellular Location Cytoplasm. Cell membrane. Note=BCR activation results in the translocation to membrane fraction
Tissue Location Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon
Research Areas
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1. J Biol Chem. 2009 Apr 10;284(15):9804-13. 2. Cancer Sci. 2008 Dec;99(12):2444-54.

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$ 385.00
Cat# AO1582a
(40 western blots)
Availability: 7-10 days
Bulk Size
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