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MMP1 Antibody

Purified Mouse Monoclonal Antibody

  • E - MMP1 Antibody AO1586a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - MMP1 Antibody AO1586a
    Figure 1: Western blot analysis using MMP1 mAb against HEK293 (1) and MMP1(AA: 24-213)-hIgGFc transfected HEK293 (2) cell lysate.
  • IHC - MMP1 Antibody AO1586a
    Figure 2: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (left) and human kidney cancer tissues (right) using MMP1 mouse mAb with DAB staining.
  • FC - MMP1 Antibody AO1586a
    Figure 3: Flow cytometric analysis of Hela cells using MMP1 mouse mAb (green) and negative control (purple).
  • IF - MMP1 Antibody AO1586a
    Figure 4: Immunofluorescence analysis of Hela cells using MMP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P03956
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6A5
Isotype IgG1
Calculated MW 54kDa
Description Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.
Immunogen Purified recombinant fragment of human MMP1 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 4312
Other Names Interstitial collagenase,, Fibroblast collagenase, Matrix metalloproteinase-1, MMP-1, 22 kDa interstitial collagenase, 27 kDa interstitial collagenase, MMP1, CLG
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/500 - 1/2000
FC~~1/200 - 1/400
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMMP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MMP1
Synonyms CLG
Function Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
Cellular Location Secreted, extracellular space, extracellular matrix
Citations (0)

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1. Arthritis Res Ther. 2009;11(6):R169. 2. FEMS Microbiol Lett. 2009 Oct;299(2):214-22.

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$ 385.00
Cat# AO1586a
(40 western blots)
Availability: 7-10 days
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