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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CD133 Antibody   

CD133 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - CD133 Antibody AO1590a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • IHC - CD133 Antibody AO1590a
    Figure 1: Immunohistochemical analysis of paraffin-embedded human breast cancer tissues (left) and human esophageal cancer tissues (right) using CD133 mouse mAb with DAB staining.
  • FC - CD133 Antibody AO1590a
    Figure 2: Flow cytometric analysis of Hela cells using CD133 mouse mAb (blue) and negative control (red).
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC, FC, E
Primary Accession O43490
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3F10
Isotype IgG1
Calculated MW 133kDa
Description This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen Synthesized peptide of human CD133.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 8842
Other Names Prominin-1, Antigen AC133, Prominin-like protein 1, CD133, PROM1, PROML1
Dilution E~~1/10000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD133 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PROM1
Synonyms PROML1
Function May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol- containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).
Cellular Location Apical cell membrane; Multi- pass membrane protein. Cell projection, microvillus membrane; Multi-pass membrane protein Cell projection, cilium, photoreceptor outer segment. Endoplasmic reticulum. Endoplasmic reticulum-Golgi intermediate compartment. Note=Found in extracellular membrane particles in various body fluids such as cerebrospinal fluid, saliva, seminal fluid and urine
Tissue Location Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells (at protein level). Expressed in adult retina by rod and cone photoreceptor cells (at protein level).
Research Areas
Citations (0)

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References

1. Stem Cells. 2009 Dec;27(12):2875-83. 2. Pancreas. 2009 Nov;38(8):e207-14.

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$ 385.00
Cat# AO1590a
Size:
Quantity:
(40 western blots)
Availability: 7-10 days
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