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AIF Antibody

Purified Mouse Monoclonal Antibody

  • E - AIF Antibody AO1595a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - AIF Antibody AO1595a
    Figure 1: Western blot analysis using AIF mAb against human AIF (AA: 1-261) recombinant protein. (Expected MW is 35.6 kDa)
  • WB - AIF Antibody AO1595a
    Figure 2: Western blot analysis using AIF mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), HepG2 (4), MOLT4 (5), C6 (6), RAJI (7), Cos7 (8) and PC-12 (9) cell lysate.
  • IHC - AIF Antibody AO1595a
    Figure 3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using AIF mouse mAb with DAB staining.
  • IHC - AIF Antibody AO1595a
    Figure 4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using AIF mouse mAb with DAB staining.
  • IHC - AIF Antibody AO1595a
    Figure 5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using AIF mouse mAb with DAB staining.
  • IHC - AIF Antibody AO1595a
    Figure 6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissues using AIF mouse mAb with DAB staining.
  • IF - AIF Antibody AO1595a
    Figure 7: Immunofluorescence analysis of NIH/3T3 cells using AIF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - AIF Antibody AO1595a
    Figure 8: Flow cytometric analysis of HepG2 cells using AIF mouse mAb (blue) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95831
Reactivity Human, Mouse, Rat, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 4E7
Isotype IgG2b
Calculated MW 67kDa
Description This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10
Immunogen Purified recombinant fragment of human AIF expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 9131
Other Names Apoptosis-inducing factor 1, mitochondrial, 1.1.1.-, Programmed cell death protein 8, AIFM1, AIF, PDCD8
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAIF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AIFM1
Synonyms AIF, PDCD8
Function Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
Cellular Location Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Note=Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region Isoform 5: Cytoplasm
Tissue Location Detected in muscle and skin fibroblasts (at protein level). Isoform 5 is frequently down-regulated in human cancers.
Research Areas
Citations (0)

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1. Apoptosis. 2009 Jun;14(6):796-808. 2. PLoS One. 2009;4(2):e4394. Epub 2009 Feb 6.

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$ 385.00
Cat# AO1595a
(40 western blots)
Availability: 7-10 days
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