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c-Jun Antibody

Purified Mouse Monoclonal Antibody

  • E - c-Jun Antibody AO1596a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - c-Jun Antibody AO1596a
    Figure 1: Western blot analysis using c-Jun mAb against human c-Jun (AA: 199-331) recombinant protein.
  • WB - c-Jun Antibody AO1596a
    Figure 2: Western blot analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.
  • IHC - c-Jun Antibody AO1596a
    Figure 3: Immunohistochemical analysis of paraffin-embedded human intima canncer tissues using c-Jun mouse mAb with DAB staining.
  • IHC - c-Jun Antibody AO1596a
    Figure 4: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using c-Jun mouse mAb with DAB staining.
  • IF - c-Jun Antibody AO1596a
    Figure 5: Immunofluorescence analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - c-Jun Antibody AO1596a
    Figure 6: Flow cytometric analysis of HepG2 cells using c-Jun mouse mAb (blue) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05412
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 5B1
Isotype IgG1
Calculated MW 43kDa
Description This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Immunogen Purified recombinant fragment of human c-Jun expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 3725
Other Names Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, JUN
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsc-Jun Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name JUN
Function Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Cellular Location Nucleus.
Research Areas
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1. J Biol Chem. 2010 Mar 12;285(11):8218-26. 2. Mol Cancer. 2010 May 19;9:111.

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$ 385.00
Cat# AO1596a
(40 western blots)
Availability: 7-10 days
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