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MMP9 Antibody

Purified Mouse Monoclonal Antibody

  • E - MMP9 Antibody AO1603a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - MMP9 Antibody AO1603a
    Figure 1: Western blot analysis using MMP9 mAb against human MMP9 (AA: 238-465) recombinant protein. (Expected MW is 50.6 kDa)
  • WB - MMP9 Antibody AO1603a
    Figure 2: Western blot analysis using MMP9 mAb against HEK293 (1), MMP7-hIgGFc transfected HEK293 (2) cell lysate and MMP9 (AA: 238-465)-hIgGFc transfected HEK293 (3) cell lysate.
  • FC - MMP9 Antibody AO1603a
    Figure 3: Flow cytometric analysis of Hela cells using MMP9 mouse mAb (blue) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14780
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5C3
Isotype IgG2a
Calculated MW 92kDa
Description Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Immunogen Purified recombinant fragment of human MMP9 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 4318
Other Names Matrix metalloproteinase-9, MMP-9,, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, MMP9, CLG4B
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMMP9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MMP9
Synonyms CLG4B
Function May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Produced by normal alveolar macrophages and granulocytes
Research Areas
Citations (0)

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1. IUBMB Life. 2009 Dec;61(12):1143-52. 2. J Biol Regul Homeost Agents. 2009 Oct-Dec;23(4):259-67.

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$ 385.00
Cat# AO1603a
(40 western blots)
Availability: 7-10 days
Bulk Size
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