|Application ||WB, IHC, FC, ICC, E|
|Description||This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.|
|Immunogen||Purified recombinant fragment of human CRK expressed in E. Coli. |
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Adapter molecule crk, Proto-oncogene c-Crk, p38, CRK|
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CRK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk- II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.|
|Cellular Location||Cytoplasm. Cell membrane. Note=Translocated to the plasma membrane upon cell adhesion.|
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Provided below are standard protocols that you may find useful for product applications.
1. Seikagaku. 2009 May;81(5):361-76. 2. Mol Cancer Res. 2009 Sep;7(9):1582-92.
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