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MCM2 Antibody

Purified Mouse Monoclonal Antibody

  • E - MCM2 Antibody AO1610a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - MCM2 Antibody AO1610a
    Figure 1: Western blot analysis using MCM2 mAb against human MCM2 (AA: 16-232) recombinant protein.(Expected MW is 50.4 kDa)
  • WB - MCM2 Antibody AO1610a
    Figure 2: Western blot analysis using MCM2 mouse mAb against PC-12 (1), Cos7 (2), NIH/3T3 (3), HepG2 (4), HEK293 (5), K562 (6), Jurkat (7), Hela (8) and MCF-7 (9) cell lysate.
  • IHC - MCM2 Antibody AO1610a
    Figure 3: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using MCM2 mouse mAb with DAB staining.
  • IHC - MCM2 Antibody AO1610a
    Figure 4: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using MCM2 mouse mAb with DAB staining.
  • FC - MCM2 Antibody AO1610a
    Figure 5: Flow cytometric analysis of Hela cells using MCM2 mouse mAb (blue) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P49736
Reactivity Human, Mouse, Rat, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 2B3
Isotype IgG1
Calculated MW 125kDa
Description The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
Immunogen Purified recombinant fragment of human MCM2 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 4171
Other Names DNA replication licensing factor MCM2,, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, MCM2, BM28, CCNL1, CDCL1, KIAA0030
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMCM2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MCM2
Synonyms BM28, CCNL1, CDCL1, KIAA0030
Function Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
Cellular Location Nucleus.
Research Areas
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1. Mol Cell. 2009 Jul 31;35(2):206-16. 2. J Cutan Pathol. 2009 Oct;36(10):1121-2.

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$ 385.00
Cat# AO1610a
(40 western blots)
Availability: 7-10 days
Bulk Size
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