|Application ||WB, IHC, FC, ICC, E|
|Reactivity||Human, Mouse, Rat, Monkey|
|Description||Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.|
|Immunogen||Purified recombinant fragment of human PHB expressed in E. Coli. |
|Formulation||Ascitic fluid containing 0.03% sodium azide.|
|Other Names||Prohibitin, PHB|
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PHB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.|
|Cellular Location||Mitochondrion inner membrane.|
|Tissue Location||Widely expressed in different tissues.|
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1. Biochem Biophys Res Commun. 2009 Dec 18;390(3):1023-8. 2. J Cell Biochem. 2009 Nov 1;108(4):926-34.
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