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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   HFE Antibody   

HFE Antibody

Purified Mouse Monoclonal Antibody

  • E - HFE Antibody AO1622a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - HFE Antibody AO1622a
    Figure 1: Western blot analysis using HFE mAb against human HFE (AA: 125-282) recombinant protein. (Expected MW is 44 kDa)
  • WB - HFE Antibody AO1622a
    Figure 2: Western blot analysis using HFE mAb against HEK293 (1) and HFE(AA: 125-282)-hIgGFc transfected HEK293 (2) cell lysate.
  • IF - HFE Antibody AO1622a
    Figure 3: Immunofluorescence analysis of HepG2 cells using HFE mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q30201
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3F1
Isotype IgG1
Calculated MW 40kDa
Description The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined. 
Immunogen Purified recombinant fragment of human HFE expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 3077
Other Names Hereditary hemochromatosis protein, HLA-H, HFE, HLAH
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHFE Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HFE
Synonyms HLAH
Function Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed in all tissues tested except brain.
Research Areas
Citations (0)

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1. Respir Med. 2009 Dec;103(12):1866-70.  2. Clin J Am Soc Nephrol. 2009 Aug;4(8):1331-7.

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$ 385.00
Cat# AO1622a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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