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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CCT2 Antibody   

CCT2 Antibody

Purified Mouse Monoclonal Antibody

  • E - CCT2 Antibody AO1624a

    Red: Control Antigen (100ng);
    Purple: Antigen (10ng);
    Green: Antigen (50ng);
    Blue: Antigen (100ng);

  • WB - CCT2 Antibody AO1624a
    Figure 1: Western blot analysis using CCT2 mAb against human CCT2 (AA: 87-290) recombinant protein. (Expected MW is 47.9 kDa)
  • WB - CCT2 Antibody AO1624a
    Figure 2: Western blot analysis using CCT2 mouse mAb against Hela (1), MCF-7 (2), Jurkat (3), T47D (4), K562 (5), A431 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
  • IF - CCT2 Antibody AO1624a
    Figure 3: Immunofluorescence analysis of 3T3-L1 cells using CCT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - CCT2 Antibody AO1624a
    Figure 4: Flow cytometric analysis of NIH/3T3 cells using CCT2 mouse mAb (blue) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P78371
Reactivity Human, Mouse, Rat, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 5B5C4
Isotype IgG1
Calculated MW 54kDa
Description The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human CCT2 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 10576
Other Names T-complex protein 1 subunit beta, TCP-1-beta, CCT-beta, CCT2, 99D8.1, CCTB
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCCT2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCT2
Synonyms 99D8.1, CCTB
Function Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
Cellular Location Cytoplasm.
Research Areas
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1. J Biol Chem. 2009 May 29;284(22):14939-48.  2. Mol Cell Proteomics. 2009 Jan;8(1):157-71.

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$ 385.00
Cat# AO1624a
(40 western blots)
Availability: 7-10 days
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