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G6PD Antibody

Purified Mouse Monoclonal Antibody

     
  • E - G6PD Antibody AO1637a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - G6PD Antibody AO1637a
    Figure 1: Western blot analysis using G6PD mAb against human G6PD (AA: 275-515) recombinant protein. (Expected MW is 53.1 kDa)

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  • WB - G6PD Antibody AO1637a
    Figure 2: Western blot analysis using G6PD mouse mAb against Hela (1), MCF-7 (2), Jurkat (3) and K562 (4) cell lysate.
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  • IHC - G6PD Antibody AO1637a
    Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using G6PD mouse mAb with DAB staining.
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  • IHC - G6PD Antibody AO1637a
    Figure 4: Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using G6PD mouse mAb with DAB staining.
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  • FC - G6PD Antibody AO1637a
    Figure 5: Flow cytometric analysis of Jurkat cells using G6PD mouse mAb (green) and negative control (red).
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession P11413
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2H7
Isotype IgG1
Calculated MW 59kDa
Description This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human G6PD expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 2539
Other Names Glucose-6-phosphate 1-dehydrogenase, G6PD, 1.1.1.49, G6PD
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsG6PD Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name G6PD
Function Catalyzes the rate-limiting step of the oxidative pentose- phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Cellular Location Cytoplasm, cytosol. Membrane; Peripheral membrane protein
Tissue Location Isoform Long is found in lymphoblasts, granulocytes and sperm
Research Areas
Citations (0)
citation

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References

1. Science. 2009 Dec 11;326(5959):1546-9. 2. Immunol Invest. 2009;38(6):551-9.

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$ 385.00
Cat# AO1637a
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