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TUBA8 Antibody

Purified Mouse Monoclonal Antibody

  • E - TUBA8 Antibody AO1651a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - TUBA8 Antibody AO1651a
    Figure 1: Western blot analysis using TUBA8 mAb against human TUBA8 (AA: 294-449) recombinant protein. (Expected MW is 50 kDa)
  • WB - TUBA8 Antibody AO1651a
    Figure 2: Western blot analysis using TUBA8 mouse mAb against rat heart (1) tissue lysate.
  • IHC - TUBA8 Antibody AO1651a
    Figure 3: Immunohistochemical analysis of paraffin-embedded medulla oblongata tissues using TUBA8 mouse mAb with DAB staining.
  • IHC - TUBA8 Antibody AO1651a
    Figure 4: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using TUBA8 mouse mAb with DAB staining.
  • IF - TUBA8 Antibody AO1651a
    Figure 5: Immunofluorescence analysis of Hela cells using TUBA8 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - TUBA8 Antibody AO1651a
    Figure 6: Flow cytometric analysis of NIH/3T3 cells using TUBA8 mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NY65
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Clone Names 2D6
Isotype IgG2b
Calculated MW 50kDa
Description This gene encodes a member of the alpha tubulin protein family. Alpha tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Mutations in this gene are associated with polymicrogyria and optic nerve hypoplasia. Alternate splicing results in multiple transcript variants. 
Immunogen Purified recombinant fragment of human TUBA8 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 1962;51807
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTUBA8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TUBA8
Synonyms TUBAL2
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Cellular Location Cytoplasm, cytoskeleton.
Tissue Location Preferentially expressed in heart, skeletal muscle and testis. Expressed at low levels in the developing brain.
Research Areas
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1. Am J Hum Genet. 2009 Nov;85(5):737-44. 2. Am J Hum Genet. 2009 Nov;85(5):628-42.

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$ 385.00
Cat# AO1651a
(40 western blots)
Availability: 7-10 days
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