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BIRC5 Antibody

Purified Mouse Monoclonal Antibody

  • E - BIRC5 Antibody AO1653a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - BIRC5 Antibody AO1653a
    Figure 1: Western blot analysis using BIRC5 mAb against human BIRC5 (AA: 1-142) recombinant protein. (Expected MW is 42 kDa)
  • WB - BIRC5 Antibody AO1653a
    Figure 2: Western blot analysis using BIRC5 mAb against HEK293 (1) and BIRC5 (AA: 1-142)-hIgGFc transfected HEK293 (2) cell lysate.
  • IHC - BIRC5 Antibody AO1653a
    Figure 3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using BIRC5 mouse mAb with DAB staining.
  • IHC - BIRC5 Antibody AO1653a
    Figure 4: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using BIRC5 mouse mAb with DAB staining.
  • IF - BIRC5 Antibody AO1653a
    Figure 5: Immunofluorescence analysis of MSCS cells using BIRC5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - BIRC5 Antibody AO1653a
    Figure 6: Flow cytometric analysis of Jurkat cells using BIRC5 mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O15392
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1H5
Isotype IgG1
Calculated MW 16kDa
Description This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human BIRC5 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 332
Other Names Baculoviral IAP repeat-containing protein 5, Apoptosis inhibitor 4, Apoptosis inhibitor survivin, BIRC5, API4, IAP4
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBIRC5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BIRC5
Synonyms API4, IAP4
Function Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis. Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Acts as an important regulator of the localization of this complex; directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules. The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. May counteract a default induction of apoptosis in G2/M phase. The acetylated form represses STAT3 transactivation of target gene promoters. May play a role in neoplasia. Inhibitor of CASP3 and CASP7. Isoform 2 and isoform 3 do not appear to play vital roles in mitosis. Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform.
Cellular Location Cytoplasm. Nucleus. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Midbody. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody. Colocalizes with AURKB at mitotic chromosomes Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1- mediated nuclear export
Tissue Location Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines.
Research Areas
Citations (0)

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1. BMC Cancer. 2010 Feb 24;10:65. 2. Int J Cancer. 2009 Oct 15;125(8):1921-5.

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$ 385.00
Cat# AO1653a
(40 western blots)
Availability: 7-10 days
Bulk Size
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