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SOX9 Antibody

Purified Mouse Monoclonal Antibody

  • E - SOX9 Antibody AO1655a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - SOX9 Antibody AO1655a
    Figure 1: Western blot analysis using SOX9 mAb against human SOX9 (AA: 147-306) recombinant protein. (Expected MW is 56 kDa)
  • WB - SOX9 Antibody AO1655a
    Figure 2: Western blot analysis using SOX9 mouse mAb against Lovo (1) and SW620 (2) cell lysate.
  • IF - SOX9 Antibody AO1655a
    Figure 3: Immunofluorescence analysis of Hela cells using SOX9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P48436
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1B11
Isotype IgG1
Calculated MW 56kDa
Description The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.
Immunogen Purified recombinant fragment of human SOX9 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 6662
Other Names Transcription factor SOX-9, SOX9
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSOX9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SOX9
Function Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00267}
Research Areas
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1. J Cell Sci. 2009 Jul 1;122(Pt 13):2191-6. 2. J Med Genet. 2009 Oct;46(10):649-56.

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$ 385.00
Cat# AO1655a
(40 western blots)
Availability: 7-10 days
Bulk Size
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