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SERPINE1 Antibody

Purified Mouse Monoclonal Antibody

  • E - SERPINE1 Antibody AO1656a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - SERPINE1 Antibody AO1656a
    Figure 1: Western blot analysis using SERPINE1 mAb against human SERPINE1 (AA: 194-316) recombinant protein. (Expected MW is 45kDa kDa)
  • WB - SERPINE1 Antibody AO1656a
    Figure 2: Western blot analysis using SERPINE1 mAb against HEK293 (1) and SERPINE1 (AA: 194-316)-hIgGFc transfected HEK293 (2) cell lysate.
  • IHC - SERPINE1 Antibody AO1656a
    Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using SERPINE1 mouse mAb with DAB staining.
  • IHC - SERPINE1 Antibody AO1656a
    Figure 4: Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using SERPINE1 mouse mAb with DAB staining.
  • FC - SERPINE1 Antibody AO1656a
    Figure 5: Flow cytometric analysis of NIH/3T3 cells using SERPINE1 mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05121
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1D5
Isotype IgG1
Calculated MW 45kDa
Description This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human SERPINE1 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5054
Other Names Plasminogen activator inhibitor 1, PAI, PAI-1, Endothelial plasminogen activator inhibitor, Serpin E1, SERPINE1, PAI1, PLANH1
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSERPINE1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms PAI1, PLANH1
Function Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.
Cellular Location Secreted.
Tissue Location Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells
Research Areas
Citations (0)

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1. Biol Pharm Bull. 2009 Apr;32(4):573-7. 2. Clin Chim Acta. 2009 Apr;402(1-2):189-92.

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$ 385.00
Cat# AO1656a
(40 western blots)
Availability: 7-10 days
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