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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PPP2R4 Antibody   

PPP2R4 Antibody

Purified Mouse Monoclonal Antibody

  • E - PPP2R4 Antibody AO1658a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - PPP2R4 Antibody AO1658a
    Figure 1: Western blot analysis using PPP2R4 mAb against human PPP2R4 (AA: 1-154) recombinant protein. (Expected MW is 41 kDa)
  • IHC - PPP2R4 Antibody AO1658a
    Figure 2: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PPP2R4 mouse mAb with DAB staining.
  • IHC - PPP2R4 Antibody AO1658a
    Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PPP2R4 mouse mAb with DAB staining.
  • IF - PPP2R4 Antibody AO1658a
    Figure 4: Immunofluorescence analysis of Hela cells using PPP2R4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - PPP2R4 Antibody AO1658a
    Figure 5: Flow cytometric analysis of MCF-7 cells using PPP2R4 mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15257
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4D9
Isotype IgG1
Calculated MW 41kDa
Description Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen Purified recombinant fragment of human PPP2R4 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5524
Other Names Serine/threonine-protein phosphatase 2A activator,, PP2A, subunit B', PR53 isoform, Phosphotyrosyl phosphatase activator, PTPA, Serine/threonine-protein phosphatase 2A regulatory subunit 4, Serine/threonine-protein phosphatase 2A regulatory subunit B', PPP2R4, PTPA
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPP2R4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPP2R4
Synonyms PTPA
Function PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine- protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+) (By similarity). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activity of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A.
Cellular Location Cytoplasm. Nucleus
Tissue Location Widely expressed.
Research Areas
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1. Stem Cells. 2010 May;28(5):874-84. 2. Mol Biol Cell. 2010 Mar 15;21(6):1140-52.

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$ 385.00
Cat# AO1658a
(40 western blots)
Availability: 7-10 days
Bulk Size
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