|Application ||WB, IHC, FC, ICC, E|
|Description||This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.|
|Immunogen||Purified recombinant fragment of human PBK expressed in E. Coli. |
|Formulation||Ascitic fluid containing 0.03% sodium azide. |
|Other Names||Lymphokine-activated killer T-cell-originated protein kinase, 188.8.131.52, Cancer/testis antigen 84, CT84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, SPK, T-LAK cell-originated protein kinase, PBK, TOPK|
IHC~~1/200 - 1/1000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PBK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.|
|Tissue Location||Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.|
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Cancer Res. 2007 Jun 1;67(11):5186-94 Cancer Sci. 2010 Feb;101(2):403-11
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