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PBK Antibody

Purified Mouse Monoclonal Antibody

  • E - PBK Antibody AO1679a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • IHC - PBK Antibody AO1679a
    Figure5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PBK mouse mAb with DAB staining.
  • WB - PBK Antibody AO1679a
    Figure 2: Western blot analysis using PBK mAb against human PBK (AA: 50-230) recombinant protein. (Expected MW is 45.8 kDa)
  • WB - PBK Antibody AO1679a
    Figure 3: Western blot analysis using PBK mouse mAb against A431 (1) cell lysate.
  • IHC - PBK Antibody AO1679a
    Figure 4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PBK mouse mAb with DAB staining.
  • IF - PBK Antibody AO1679a
    Figure 6: Immunofluorescence analysis of Hela cells using PBK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - PBK Antibody AO1679a
    Figure 7: Flow cytometric analysis of Hela cells using PBK mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96KB5
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2C8
Isotype IgG2b
Calculated MW 36kDa
Description This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.
Immunogen Purified recombinant fragment of human PBK expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 55872
Other Names Lymphokine-activated killer T-cell-originated protein kinase,, Cancer/testis antigen 84, CT84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, SPK, T-LAK cell-originated protein kinase, PBK, TOPK
Dilution E~~1/10000
IHC~~1/200 - 1/1000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPBK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PBK
Synonyms TOPK
Function Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Tissue Location Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
Research Areas
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Cancer Res. 2007 Jun 1;67(11):5186-94 Cancer Sci. 2010 Feb;101(2):403-11

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$ 385.00
Cat# AO1679a
(40 western blots)
Availability: 7-10 days
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