|Application ||WB, IHC, FC, E|
|Description||There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described.|
|Immunogen||Purified recombinant fragment of human ALPL expressed in E. Coli. |
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Other Names||Alkaline phosphatase, tissue-nonspecific isozyme, AP-TNAP, TNSALP, 184.108.40.206, Alkaline phosphatase liver/bone/kidney isozyme, ALPL|
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ALPL Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||This isozyme may play a role in skeletal mineralization.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor|
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J Rheumatol. 2009 Dec;36(12):2758-65. Calcif Tissue Int. 2009 Sep;85(3):228-34.
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