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FN1 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - FN1 Antibody AO1689a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - FN1 Antibody AO1689a
    Figure 1: Western blot analysis using FN1 mAb against human FN1 (AA: 1965-2176) recombinant protein. (Expected MW is 49.6 kDa)
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  • WB - FN1 Antibody AO1689a
    Figure 2: Western blot analysis using FN1 mAb against HEK293 (1) and FN1 (AA: 1965-2176)-hIgGFc transfected HEK293 (2) cell lysate.
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  • IHC - FN1 Antibody AO1689a
    Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using FN1 mouse mAb with DAB staining.
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  • IHC - FN1 Antibody AO1689a
    Figure 4: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using FN1 mouse mAb with DAB staining.
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  • FC - FN1 Antibody AO1689a
    Figure 5: Flow cytometric analysis of HeLa cells using FN1 mouse mAb (green) and negative control (red).
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession P02751
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2F4
Isotype IgG1
Calculated MW 262.6kDa
Description This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
Immunogen Purified recombinant fragment of human FN1 expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 2335
Other Names Fibronectin, FN, Cold-insoluble globulin, CIG, Anastellin, Ugl-Y1, Ugl-Y2, Ugl-Y3, FN1, FN
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFN1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FN1 (HGNC:3778)
Synonyms FN
Function Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed:3024962, PubMed:3900070, PubMed:3593230, PubMed:7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed:34089617).
Cellular Location Secreted, extracellular space, extracellular matrix. Secreted {ECO:0000250|UniProtKB:P11276}
Tissue Location Expressed in the inner limiting membrane and around blood vessels in the retina (at protein level) (PubMed:29777959) Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine (PubMed:17614963).
Research Areas
Citations (0)
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References

Pancreas. 2009 Oct;38(7):804-10. J Biol Chem. 2009 Sep 18;284(38):25879-88.

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$ 385.00
Cat# AO1689a
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