HSF4 Antibody
Purified Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, FC, E |
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Primary Accession | Q9ULV5 |
Reactivity | Human |
Host | Mouse |
Clonality | Monoclonal |
Clone Names | 2E7 |
Isotype | IgG1 |
Calculated MW | 53kDa |
Description | Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described. |
Immunogen | Purified recombinant fragment of human HSF4 expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Gene ID | 3299 |
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Other Names | Heat shock factor protein 4, HSF 4, hHSF4, Heat shock transcription factor 4, HSTF 4, HSF4 |
Dilution | E~~1/10000 WB~~1/500 - 1/2000 FC~~1/200 - 1/400 |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | HSF4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | HSF4 |
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Function | Heat-shock transcription factor that specifically binds heat shock promoter elements (HSE) (PubMed:22587838, PubMed:23507146). Required for denucleation and organelle rupture and degradation that occur during eye lens terminal differentiation, when fiber cells that compose the lens degrade all membrane-bound organelles in order to provide lens with transparency to allow the passage of light (By similarity). In this process, may regulate denucleation of lens fiber cells in part by activating DNASE2B transcription (By similarity). May be involved in DNA repair through the transcriptional regulation of RAD51 (PubMed:22587838). May up-regulate p53/TP53 protein in eye lens fiber cells, possibly through protein stabilization (PubMed:28981088). In the eye lens, controls the expression of alpha-crystallin B chain/CRYAB and consequently may be involved in the regulation of lysosomal acidification (By similarity). |
Cellular Location | Nucleus. |
Tissue Location | Expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues |
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Provided below are standard protocols that you may find useful for product applications.
References
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Apr;26(4):325-8. Am J Hum Genet. 2009 Nov;85(5):628-42.
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