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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   HSF4 Antibody   

HSF4 Antibody

Purified Mouse Monoclonal Antibody

  • E - HSF4 Antibody AO1700a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - HSF4 Antibody AO1700a
    Figure 1: Western blot analysis using HSF4 mAb against human HSF4 (AA: 245-411) recombinant protein. (Expected MW is 42.9 kDa)
  • FC - HSF4 Antibody AO1700a
    Figure 2: Flow cytometric analysis of HeLa cells using HSF4 mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9ULV5
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2E7
Isotype IgG1
Calculated MW 53kDa
Description Heat-shock transcription factors (HSFs) activate heat-shock response genes under conditions of heat or other stresses. HSF4 lacks the carboxyl-terminal hydrophobic repeat which is shared among all vertebrate HSFs and has been suggested to be involved in the negative regulation of DNA binding activity. Two alternatively spliced transcripts encoding distinct isoforms and possessing different transcriptional activity have been described.
Immunogen Purified recombinant fragment of human HSF4 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 3299
Other Names Heat shock factor protein 4, HSF 4, hHSF4, Heat shock transcription factor 4, HSTF 4, HSF4
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSF4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSF4
Function DNA-binding protein that specifically binds heat shock promoter elements (HSE). Isoform HSF4A represses transcription while the isoform HSF4B activates transcription.
Cellular Location Nucleus.
Tissue Location Expressed in heart, skeletal muscle, eye and brain, and at much lower levels in some other tissues
Research Areas
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Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Apr;26(4):325-8. Am J Hum Genet. 2009 Nov;85(5):628-42.

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$ 385.00
Cat# AO1700a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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