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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RAP1A Antibody   

RAP1A Antibody

Purified Mouse Monoclonal Antibody

  • E - RAP1A Antibody AO1704a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - RAP1A Antibody AO1704a
    Figure 1: Western blot analysis using RAP1A mAb against human RAP1A (AA: 28-180) recombinant protein. (Expected MW is 43.2 kDa)
  • WB - RAP1A Antibody AO1704a
    Figure 2: Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - RAP1A Antibody AO1704a
    Figure 3: Flow cytometric analysis of A431 cells using RAP1A mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P62834
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5F8
Isotype IgG1
Calculated MW 21kDa
Description The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.
Immunogen Purified recombinant fragment of human RAP1A expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5906
Other Names Ras-related protein Rap-1A, C21KG, G-22K, GTP-binding protein smg p21A, Ras-related protein Krev-1, RAP1A, KREV1
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAP1A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RAP1A
Synonyms KREV1
Function Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. Together with ITGB1BP1, regulates KRIT1 localization to microtubules and membranes. Plays a role in nerve growth factor (NGF)-induced neurite outgrowth. Plays a role in the regulation of embryonic blood vessel formation. Involved in the establishment of basal endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR expression at endothelial cell-cell junctions.
Cellular Location Cell membrane; Lipid-anchor. Cytoplasm. Cytoplasm, perinuclear region. Cell junction. Early endosome. Note=Recruited from early endosome to late endosome compartment after nerve growth factor (NGF) stimulation Localized with RAPGEF2 at cell-cell junctions (By similarity) Colocalized with RAPGEF2 in the perinuclear region.
Research Areas
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Mol Biol Cell. 2009 Apr;20(7):1916-25. J Cell Sci. 2009 Aug 15;122(Pt 16):2996-3004.

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$ 385.00
Cat# AO1704a
(40 western blots)
Availability: 7-10 days
Bulk Size
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