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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   HSP90AA1 Antibody   

HSP90AA1 Antibody

Purified Mouse Monoclonal Antibody

  • E - HSP90AA1 Antibody AO1706a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - HSP90AA1 Antibody AO1706a
    Figure 1: Western blot analysis using HSP90AA1 mAb against human HSP90AA1 (AA: 275-484) recombinant protein. (Expected MW is 50.5 kDa)
  • WB - HSP90AA1 Antibody AO1706a
    Figure 2: Western blot analysis using HSP90AA1 mouse mAb against NIH3T3 (1), HeLa (2), HCT116(3), HL-60 (4) and C0S7 (5) cell lysate.
  • IHC - HSP90AA1 Antibody AO1706a
    Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using HSP90AA1 mouse mAb with DAB staining.
  • IHC - HSP90AA1 Antibody AO1706a
    Figure 4: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using HSP90AA1 mouse mAb with DAB staining.
  • FC - HSP90AA1 Antibody AO1706a
    Figure 5: Flow cytometric analysis of HeLa cells using HSP90AA1 mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P07900
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 5G5
Isotype IgG1
Calculated MW 90kDa
Description HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).
Immunogen Purified recombinant fragment of human HSP90AA1 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 3320
Other Names Heat shock protein HSP 90-alpha, Heat shock 86 kDa, HSP 86, HSP86, Lipopolysaccharide-associated protein 2, LAP-2, LPS-associated protein 2, Renal carcinoma antigen NY-REN-38, HSP90AA1, HSP90A, HSPC1, HSPCA
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHSP90AA1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HSP90AA1
Synonyms HSP90A, HSPC1, HSPCA
Function Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes.
Cellular Location Cytoplasm. Melanosome. Cell membrane. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Research Areas
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Mol Biol Cell. 2010 May 1;21(9):1462-9. Mol Cancer Ther. 2009 Dec;8(12):3296-306.

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$ 385.00
Cat# AO1706a
(40 western blots)
Availability: 7-10 days
Bulk Size
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