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MAP1LC3B Antibody

Purified Mouse Monoclonal Antibody

  • E - MAP1LC3B Antibody AO1713a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - MAP1LC3B Antibody AO1713a
    Figure 1: Western blot analysis using MAP1LC3B mAb against human MAP1LC3B (AA: 1-125) recombinant protein. (Expected MW is 40.2 kDa)
  • WB - MAP1LC3B Antibody AO1713a
    Figure 2: Western blot analysis using MAP1LC3B mAb against HEK293 (1) and MAP1LC3B (AA: 1-125)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - MAP1LC3B Antibody AO1713a
    Figure 3: Flow cytometric analysis of HeLa cells using MAP1LC3B mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9GZQ8
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 5H12
Isotype IgG1
Calculated MW 14.6kDa
Description The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Immunogen Purified recombinant fragment of human MAP1LC3B expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 81631
Other Names Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1LC3B, MAP1ALC3
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAP1LC3B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms MAP1ALC3
Function Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
Cellular Location Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes Localizes also to discrete punctae along the ciliary axoneme (By similarity).
Tissue Location Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver
Research Areas
Citations (0)

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1. J Clin Lab Anal. 2009;23(4):249-58. 2. Ai Zheng. 2008 Jan;27(1):25-9.

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$ 385.00
Cat# AO1713a
(40 western blots)
Availability: 7-10 days
Bulk Size
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