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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NKX2.2 Antibody   

NKX2.2 Antibody

Purified Mouse Monoclonal Antibody

  • E - NKX2.2 Antibody AO1717a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - NKX2.2 Antibody AO1717a
    Figure 1: Western blot analysis using NKX2.2 mAb against human NKX2.2 recombinant protein.(Expected MW is 22 kDa)
  • FC - NKX2.2 Antibody AO1717a
    Figure 2: Flow cytometric analysis of MCF-7 cells using NKX2.2 mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O95096
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3E4
Isotype IgG1
Calculated MW 30.1kDa
Description The protein encoded by this gene contains a homeobox domain and may be involved in the morphogenesis of the central nervous system. This gene is found on chromosome 20 near NKX2-4, and these two genes appear to be duplicated on chromosome 14 in the form of TITF1 and NKX2-8. The encoded protein is likely to be a nuclear transcription factor.
Immunogen Purified recombinant fragment of human NKX2.2 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 4821
Other Names Homeobox protein Nkx-2.2, Homeobox protein NK-2 homolog B, NKX2-2, NKX2.2, NKX2B
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNKX2.2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NKX2-2
Synonyms NKX2.2, NKX2B
Function Acts as a transcriptional activator. Required for the maintenance of NEUROD1 expression in the horomone-producing endocrine cells of the pancreas. May be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Associates with chromatin at the NEUROD1 promoter region. Binds to a subset of consensus elements within the NEUROD1 promoter (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00108}
Research Areas
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1. J Surg Res. 2010 Sep;163(1):47-51 2. Endocr Relat Cancer. 2009 Mar;16(1):267-79.

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$ 385.00
Cat# AO1717a
(40 western blots)
Availability: 7-10 days
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