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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NOS2 Antibody   

NOS2 Antibody

Purified Mouse Monoclonal Antibody

  • E - NOS2 Antibody AO1718a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - NOS2 Antibody AO1718a
    Figure 1: Western blot analysis using NOS2 mAb against human NOS2 (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa)
  • WB - NOS2 Antibody AO1718a
    Figure 2: Western blot analysis using NOS2 mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5)and MCF-7 (6) cell lysate.
  • IHC - NOS2 Antibody AO1718a
    Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NOS2 mouse mAb with DAB staining.
  • IHC - NOS2 Antibody AO1718a
    Figure 4: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using NOS2 mouse mAb with DAB staining.
  • FC - NOS2 Antibody AO1718a
    Figure 5: Flow cytometric analysis of MCF-7 cells using NOS2 mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35228
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 4E5
Isotype IgG1
Calculated MW 131kDa
Description Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
Immunogen Purified recombinant fragment of human NOS2 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 4843
Other Names Nitric oxide synthase, inducible,, Hepatocyte NOS, HEP-NOS, Inducible NO synthase, Inducible NOS, iNOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, NOS2, NOS2A
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNOS2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NOS2
Synonyms NOS2A
Function Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. As component of the iNOS- S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys- 247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM.
Tissue Location Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets
Research Areas
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1. Pediatr Allergy Immunol. 2010 Dec;21(8):1151-6. 2. J Biol Chem. 2010 Dec 31;285(53):41422-31.

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$ 385.00
Cat# AO1718a
(40 western blots)
Availability: 7-10 days
Bulk Size
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