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GPC3 Antibody

Purified Mouse Monoclonal Antibody

  • E - GPC3 Antibody AO1722a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - GPC3 Antibody AO1722a
    Figure 1: Western blot analysis using GPC3 mAb against human GPC3 (AA: 55-200) recombinant protein. (Expected MW is 28.5 kDa)
  • WB - GPC3 Antibody AO1722a
    Figure 2: Western blot analysis using GPC3 mouse mAb against HepG2 (1), HEK293 (2), Jurkat (3), SK-N-SH (4), PC-12 (5), F9 (6)and Mouse liver (7) cell lysate.
  • IHC - GPC3 Antibody AO1722a
    Figure 3: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using GPC3 mouse mAb with DAB staining.
  • IHC - GPC3 Antibody AO1722a
    Figure 4: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GPC3 mouse mAb with DAB staining.
  • IF - GPC3 Antibody AO1722a
    Figure 5: Immunofluorescence analysis of HeLa cells using GPC3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - GPC3 Antibody AO1722a
    Figure 6: Flow cytometric analysis of Jurkat cells using GPC3 mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P51654
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 9C2
Isotype IgG1
Calculated MW 65.5kDa
Description Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.
Immunogen Purified recombinant fragment of human GPC3 expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 2719
Other Names Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7, Secreted glypican-3, GPC3, OCI5
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGPC3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GPC3
Synonyms OCI5
Function Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side
Tissue Location Highly expressed in lung, liver and kidney.
Research Areas
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1. Int J Cancer. 2010 Mar 15;126(6):1291-301. 2. Cancer Biol Ther. 2009 Dec;8(24):2329-38.

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$ 385.00
Cat# AO1722a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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