|Application ||WB, IHC, FC, ICC, E|
|Description||Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.|
|Immunogen||Purified recombinant fragment of human GPC3 expressed in E. Coli. |
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Other Names||Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7, Secreted glypican-3, GPC3, OCI5|
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GPC3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side|
|Tissue Location||Highly expressed in lung, liver and kidney.|
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Provided below are standard protocols that you may find useful for product applications.
1. Int J Cancer. 2010 Mar 15;126(6):1291-301. 2. Cancer Biol Ther. 2009 Dec;8(24):2329-38.
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