|Application ||IHC, FC, ICC, E|
|Description||Maternal embryonic leucine-zipper kinase (MELK) is a key regulator of survival of stemlike GBM cells in vitro. MELK expression is increased in breast cancer tissue and this increase is also associated with poor patient survival, as predicted for a candidate oncogene.|
|Immunogen||Synthesized peptide of human MELK(AA: 637-651:C-VYKRLVEDILSSCKV). |
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Other Names||Maternal embryonic leucine zipper kinase, hMELK, 18.104.22.168, Protein kinase Eg3, pEg3 kinase, Protein kinase PK38, hPK38, Tyrosine-protein kinase MELK, 22.214.171.124, MELK, KIAA0175|
IHC~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MELK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.|
|Cellular Location||Cell membrane; Peripheral membrane protein|
|Tissue Location||Expressed in placenta, kidney, thymus, testis, ovary and intestine.|
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1. Neuro Oncol. 2011 Jun;13(6):622-34. 2. Breast Cancer Res. 2009;11(4):R60.
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