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DCX Antibody

Purified Mouse Monoclonal Antibody

  • E - DCX Antibody AO1731a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - DCX Antibody AO1731a
    Figure 1: Western blot analysis using DCX mAb against human DCX recombinant protein. (Expected MW is 34.1 kDa)
  • WB - DCX Antibody AO1731a
    Figure 2: Western blot analysis using DCX mouse mAb against Mouse heart (1) lysate.
  • IHC - DCX Antibody AO1731a
    Figure 3: Immunohistochemical analysis of paraffin-embedded brain tissue tissues using DCX mouse mAb with DAB staining.
  • IHC - DCX Antibody AO1731a
    Figure 4: Immunohistochemical analysis of paraffin-embedded kidney cancer tissues using DCX mouse mAb with DAB staining.
  • IF - DCX Antibody AO1731a
    Figure 5: Immunofluorescence analysis of HepG2 cells using DCX mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • FC - DCX Antibody AO1731a
    Figure 6: Flow cytometric analysis of SK-N-SH cells using DCX mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43602
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2G5
Isotype IgG1
Calculated MW 49.3kDa
Description This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex"syndrome) in females and lissencephaly ("smooth brain"syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human DCX (AA: 362-411) expressed in E. Coli.

Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 1641
Other Names Neuronal migration protein doublecortin, Doublin, Lissencephalin-X, Lis-X, DCX, DBCN, LISX
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDCX Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DCX
Synonyms DBCN, LISX
Function Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Cellular Location Cytoplasm. Cell projection. Note=Localizes at neurite tips.
Tissue Location Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas
Research Areas
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1.FASEB J. 2009 Dec;23(12):4276-87. 2.J Cell Biol. 2010 Nov 1;191(3):463-70.

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$ 385.00
Cat# AO1731a
(40 western blots)
Availability: 7-10 days
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