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E2F1 Antibody

Purified Mouse Monoclonal Antibody

  • E - E2F1 Antibody AO1734a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - E2F1 Antibody AO1734a
    Figure 1: Western blot analysis using E2F1 mAb against human E2F1 recombinant protein. (Expected MW is 42.7 kDa)
  • WB - E2F1 Antibody AO1734a
    Figure 2: Western blot analysis using E2F1 mouse mAb against HeLa (1), SK-N-SH (2), and NIH3T3 (3) cell lysate.
  • IHC - E2F1 Antibody AO1734a
    Figure 3: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using E2F1 mouse mAb with DAB staining.
  • IHC - E2F1 Antibody AO1734a
    Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using E2F1 mouse mAb with DAB staining.
  • FC - E2F1 Antibody AO1734a
    Figure 5: Flow cytometric analysis of HeLa cells using E2F1 mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q01094
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 8G9
Isotype IgG1
Calculated MW 46.9kDa
Description The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Immunogen Purified recombinant fragment of human E2F1 (AA: 69-223) expressed in E. Coli.

Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 1869
Other Names Transcription factor E2F1, E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1, E2F1, RBBP3
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsE2F1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name E2F1
Synonyms RBBP3
Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812).
Cellular Location Nucleus.
Citations (0)

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1.Cancer Res. 2010 Dec 1;70(23):9711-20. 2.Mol Cancer Ther. 2010 May;9(5):1265-73.

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$ 385.00
Cat# AO1734a
(40 western blots)
Availability: 7-10 days
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