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DLL4 Antibody

Purified Mouse Monoclonal Antibody

  • E - DLL4 Antibody AO1736a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - DLL4 Antibody AO1736a
    Figure 1: Western blot analysis using DLL4 mAb against human DLL4 recombinant protein. (Expected MW is 39.2 kDa)
  • WB - DLL4 Antibody AO1736a
    Figure 2: Western blot analysis using DLL4 mAb against HEK293 (1) and DLL4 (AA: 313-439)-hIgGFc transfected HEK293 (2) cell lysate.
  • IHC - DLL4 Antibody AO1736a
    Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using DLL4 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NR61
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4A11F8
Isotype IgG1
Calculated MW 74.6kDa
Description This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.
Immunogen Purified recombinant fragment of human DLL4 (AA: 313-439) expressed in E. Coli.

Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 54567
Other Names Delta-like protein 4, Drosophila Delta homolog 4, Delta4, DLL4
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDLL4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DLL4
Function Involved in the Notch signaling pathway as Notch ligand. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation is required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types. During spinal cord neurogenesis, inhibits V2a interneuron fate.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed in vascular endothelium.
Research Areas
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1.Am J Pathol. 2010 Apr;176(4):2019-28. 2.Blood. 2010 Sep 30;116(13):2385-94.

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$ 385.00
Cat# AO1736a
(40 western blots)
Availability: 7-10 days
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