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CD5 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - CD5 Antibody AO1750a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

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  • WB - CD5 Antibody AO1750a
    Figure 1: Western blot analysis using CD5 mAb against human CD5 recombinant protein. (Expected MW is 48.3 kDa)
    detail
  • WB - CD5 Antibody AO1750a
    Figure 2: Western blot analysis using CD5 mouse mAb against K562 (1), Jurkat (2), Raji (3), and MOLT4 (4) cell lysate.
    detail
  • FC - CD5 Antibody AO1750a
    Figure 3: Flow cytometric analysis of Jurkat cells using CD5 mouse mAb (green) and negative control (red).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P06127
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6A11
Isotype IgG1
Calculated MW 54.6kDa
Description The human cd5 gene has two alternative exons 1: exon 1A (E1A) which encodes the full-length (FL) CD5 protein and exon 1B (E1B) which encodes a truncated (TR) isoform. The FL variant of CD5 protein is translocated to the plasma membrane, while its TR variant is retained in the cytoplasm.
Immunogen Purified recombinant fragment of human CD5 (AA: 27-233) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 921
Other Names T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, CD5, CD5, LEU1
Dilution E~~1/10000
WB~~1/250
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD5
Synonyms LEU1
Function May act as a receptor in regulating T-cell proliferation.
Cellular Location Cell membrane; Single-pass type I membrane protein
Research Areas
Citations (0)
citation

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References

1.Crit Rev Immunol. 2011;31(1):31-42.2.Blood. 2011 Dec 1;118(23):6107-14.

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$ 385.00
Cat# AO1750a
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