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TWF1 Antibody

Purified Mouse Monoclonal Antibody

  • E - TWF1 Antibody AO1756a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - TWF1 Antibody AO1756a
    Figure 1: Western blot analysis using TWF1 mAb against human TWF1 recombinant protein. (Expected MW is 31.1 kDa)
  • IHC - TWF1 Antibody AO1756a
    Figure 2: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TWF1 mouse mAb with DAB staining.
  • WB - TWF1 Antibody AO1756a
    Figure 2: Western blot analysis using TWF1 mAb against HEK293 (1) and TWF1 (AA: 335-384)-hIgGFc transfected HEK293 (2) cell lysate.
  • IHC - TWF1 Antibody AO1756a
    Figure 2: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TWF1 mouse mAb with DAB staining.
  • IF - TWF1 Antibody AO1756a
    Figure 3: Immunofluorescence analysis of HeLa cells using TWF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • FC - TWF1 Antibody AO1756a
    Figure 4: Flow cytometric analysis of HeLa cells using TWF1 mouse mAb (green) and negative control (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q12792
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7C11
Isotype IgG1
Calculated MW 40.3kDa
Description This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.
Immunogen Purified recombinant fragment of human TWF1 (AA: 335-384 ) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5756
Other Names Twinfilin-1, Protein A6, Protein tyrosine kinase 9, TWF1, PTK9
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTWF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TWF1
Synonyms PTK9
Function Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (By similarity).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Note=Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts (By similarity).
Tissue Location Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver
Research Areas
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1.Cancer Epidemiol Biomarkers Prev. 2010 May;19(5):1356-61.2.Cancer Epidemiol Biomarkers Prev. 2009 May;18(5):1651-8.

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$ 385.00
Cat# AO1756a
(40 western blots)
Availability: 7-10 days
Bulk Size
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