|Application ||WB, FC, E|
|Description||Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand.|
|Immunogen||Purified recombinant fragment of human FZD5 (AA:151-217) expressed in E. Coli.|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Other Names||Frizzled-5, Fz-5, hFz5, FzE5, FZD5, C2orf31|
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||FZD5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta- catenin pathway.|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein Note=Localized at the plasma membrane and also found at the Golgi|
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1.J Biol Chem. 2009 Sep 25;284(39):26716-24.2.Int J Oncol. 2007 Mar;30(3):751-5.
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