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ILK Antibody

Purified Mouse Monoclonal Antibody

  • E - ILK Antibody AO1763a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ILK Antibody AO1763a
    Figure 1: Western blot analysis using ILK mAb against human ILK recombinant protein. (Expected MW is 42.7 kDa)
  • WB - ILK Antibody AO1763a
    Figure 2: Western blot analysis using ILK mouse mAb against Jurkat (1), NIH3T3 (2), HeLa (3), PC-12 (4), C6 (5), COS7 (6), Raji (7), K562 (8) and MCF-7 (9) cell lysate.
  • FC - ILK Antibody AO1763a
    Figure 3: Flow cytometric analysis of Jurkat cells using ILK mouse mAb (green) and negative control (red).
  • IHC - ILK Antibody AO1763a
    Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using ILK mouse mAb with DAB staining.
  • IHC - ILK Antibody AO1763a
    Figure 5: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ILK mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13418
Reactivity Human, Mouse, Monkey
Host Mouse
Clonality Monoclonal
Clone Names 3A9
Isotype IgG1
Calculated MW 51.4kDa
Description Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Immunogen Purified recombinant fragment of human ILK (AA: 97-244) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 3611
Other Names Integrin-linked protein kinase,, 59 kDa serine/threonine-protein kinase, ILK-1, ILK-2, p59ILK, ILK, ILK1, ILK2
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsILK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ILK
Synonyms ILK1, ILK2
Function Receptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
Cellular Location Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium. Cytoplasm, myofibril, sarcomere
Tissue Location Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver
Research Areas
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1.Surgery. 2011 Aug;150(2):162-8.2.Int J Cancer. 2012 Feb 1;130(3):521-31.

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$ 385.00
Cat# AO1763a
(40 western blots)
Availability: 7-10 days
Bulk Size
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