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MARK3 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - MARK3 Antibody AO1766a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

    detail
  • WB - MARK3 Antibody AO1766a
    Figure 1: Western blot analysis using MARK3 mAb against human MARK3 recombinant protein. (Expected MW is 40.8 kDa)
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  • WB - MARK3 Antibody AO1766a
    Figure 2: Western blot analysis using MARK3 mouse mAb against HeLa (1), SK-N-SH (2), K562 (3), HCT116 (4), HEK293 (5), 3T3L1 (6), NIH3T3 (7), Jurkat (8), and A431 (9) cell lysate.
    detail
  • FC - MARK3 Antibody AO1766a
    Figure 3: Flow cytometric analysis of SK-N-SH cells using MARK3 mouse mAb (green) and negative control (red).
    detail
  • IHC - MARK3 Antibody AO1766a
    Figure 4: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using MARK3 mouse mAb with DAB staining.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession P27448
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 2G12
Isotype IgG1
Calculated MW 87kDa
Description The protein encoded by this gene is activated by phosphorylation and in turn is involved in the phosphorylation of tau proteins MAP2 and MAP4. Several transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human MARK3 (AA: 435-658) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 4140
Other Names MAP/microtubule affinity-regulating kinase 3, 2.7.11.1, C-TAK1, cTAK1, Cdc25C-associated protein kinase 1, ELKL motif kinase 2, EMK-2, Protein kinase STK10, Ser/Thr protein kinase PAR-1, Par-1a, Serine/threonine-protein kinase p78, MARK3, CTAK1, EMK2
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMARK3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MARK3
Synonyms CTAK1, EMK2
Function Serine/threonine-protein kinase (PubMed:16822840, PubMed:16980613, PubMed:23666762). Involved in the specific phosphorylation of microtubule-associated proteins for MAP2 and MAP4. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed:23666762). Phosphorylates CDC25C on 'Ser-216' (PubMed:12941695). Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus (PubMed:16980613). Regulates localization and activity of MITF by mediating its phosphorylation, promoting subsequent interaction between MITF and 14-3-3 and retention in the cytosol (PubMed:16822840). Negatively regulates the Hippo signaling pathway and antagonizes the phosphorylation of LATS1. Cooperates with DLG5 to inhibit the kinase activity of STK3/MST2 toward LATS1 (PubMed:28087714). Phosphorylates PKP2 and KSR1 (PubMed:12941695).
Cellular Location Cell membrane; Peripheral membrane protein. Cell projection, dendrite. Cytoplasm
Tissue Location Ubiquitous.
Research Areas
Citations (0)
citation

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References

1.Biochem Biophys Res Commun. 2010 Apr 16;394(4):890-5.2.Biochem J. 2008 Apr 15;411(2):249-60.

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$ 385.00
Cat# AO1766a
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