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STAT5A Antibody

Purified Mouse Monoclonal Antibody

  • E - STAT5A Antibody AO1770a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - STAT5A Antibody AO1770a
    Figure 1: Western blot analysis using STAT5A mAb against human STAT5A recombinant protein. (Expected MW is 49.3 kDa)
  • WB - STAT5A Antibody AO1770a
    Figure 2: Western blot analysis using STAT5A mouse mAb against K562 (1), MOLT4 (2), HeLa (3), Jurkat (4), and A431 (5) cell lysate.
  • IF - STAT5A Antibody AO1770a
    Figure 3: Immunofluorescence analysis of Hela cells using STAT5A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • FC - STAT5A Antibody AO1770a
    Figure 4: Flow cytometric analysis of K562 cells using STAT5A mouse mAb (green) and negative control (red).
  • IHC - STAT5A Antibody AO1770a
    Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using STAT5A mouse mAb with DAB staining.
  • IHC - STAT5A Antibody AO1770a
    Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using STAT5A mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P42229
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6D4
Isotype IgG1
Calculated MW 90.6kDa
Description The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells.
Immunogen Purified recombinant fragment of human STAT5A (AA: 583-794 ) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 6776
Other Names Signal transducer and activator of transcription 5A, STAT5A, STAT5
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSTAT5A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms STAT5
Function Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
Cellular Location Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation
Research Areas
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1.Cancer Res. 2011 May 15;71(10):3720-31. 2.Blood. 2011 Mar 24;117(12):3409-20.

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$ 385.00
Cat# AO1770a
(40 western blots)
Availability: 7-10 days
Bulk Size
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