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CD59 Antibody

Purified Mouse Monoclonal Antibody

  • E - CD59 Antibody AO1775a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - CD59 Antibody AO1775a
    Figure 1: Western blot analysis using CD59 mAb against human CD59 recombinant protein. (Expected MW is 34.7 kDa)
  • WB - CD59 Antibody AO1775a
    Figure 2: Western blot analysis using CD59 mAb against HEK293 (1) and CD59 (AA: 31-111)-hIgGFc transfected HEK293 (2) cell lysate.
  • IF - CD59 Antibody AO1775a
    Figure 3: Immunofluorescence analysis of MCF-7 cells using CD59 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • IF - CD59 Antibody AO1775a
    Figure 4: Immunofluorescence analysis of Hela cells using CD59 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - CD59 Antibody AO1775a
    Figure 5: Flow cytometric analysis of HeLa cells using CD59 mouse mAb (green) and negative control (red).
  • IHC - CD59 Antibody AO1775a
    Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD59 mouse mAb with DAB staining.
  • IHC - CD59 Antibody AO1775a
    Figure 7: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using CD59 mouse mAb with DAB staining.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P13987
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8D2B8
Isotype IgG1
Calculated MW 14.2kDa
Description This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.
Immunogen Purified recombinant fragment of human CD59 (AA: 31-111) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 966
Other Names CD59 glycoprotein, 1F5 antigen, 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-inhibitory protein, MAC-IP, MEM43 antigen, Membrane attack complex inhibition factor, MACIF, Membrane inhibitor of reactive lysis, MIRL, Protectin, CD59, CD59, MIC11, MIN1, MIN2, MIN3, MSK21
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/50 -1/200
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD59 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD59
Synonyms MIC11, MIN1, MIN2, MIN3, MSK21
Function Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Note=Soluble form found in a number of tissues
Citations (0)

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1.Cell Immunol. 2010;265(2):127-32. 2.Chin Med J (Engl). 2009 Sep 20;122(18):2123-8.

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$ 385.00
Cat# AO1775a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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