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KEAP1 Antibody

Purified Mouse Monoclonal Antibody

  • E - KEAP1 Antibody AO1778a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - KEAP1 Antibody AO1778a
    Figure 1: Western blot analysis using KEAP1 mAb against human KEAP1 recombinant protein. (Expected MW is 52.7 kDa)
  • WB - KEAP1 Antibody AO1778a
    Figure 2: Western blot analysis using KEAP1 mouse mAb against NIH3T3 (1), and A549 (2) cell lysate.
  • IF - KEAP1 Antibody AO1778a
    Figure 3: Immunofluorescence analysis of HeLa cells using KEAP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • FC - KEAP1 Antibody AO1778a
    Figure 4: Flow cytometric analysis of HepG2 cells using KEAP1 mouse mAb (green) and negative control (purple).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14145
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Clone Names 1F10B6
Isotype IgG1
Calculated MW 69.7kDa
Description This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Immunogen Purified recombinant fragment of human KEAP1 (AA: 380-624) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 9817
Other Names Kelch-like ECH-associated protein 1, Cytosolic inhibitor of Nrf2, INrf2, Kelch-like protein 19, KEAP1, INRF2, KIAA0132, KLHL19
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKEAP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KEAP1
Synonyms INRF2, KIAA0132, KLHL19
Function Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
Cellular Location Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and nucleus
Tissue Location Broadly expressed, with highest levels in skeletal muscle.
Research Areas
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1.Cell Signal. 2010 Nov;22(11):1645-54. 2.Mol Cancer Ther. 2010 Feb;9(2):336-46.

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$ 385.00
Cat# AO1778a
(40 western blots)
Availability: 7-10 days
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