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PPY Antibody

Purified Mouse Monoclonal Antibody

     
  • E - PPY Antibody AO1780a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

    detail
  • WB - PPY Antibody AO1780a
    Figure 1: Western blot analysis using PPY mAb against human PPY recombinant protein. (Expected MW is 35.9 kDa)
    detail
  • FC - PPY Antibody AO1780a
    Figure 2: Flow cytometric analysis of HepG2 cells using PPY mouse mAb (green) and negative control (purple).
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P01298
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2G5D12
Isotype IgG1
Calculated MW 10.4kDa
Description This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain.
Immunogen Purified recombinant fragment of human PPY (AA: 1-95) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 5539
Other Names Pancreatic prohormone, Pancreatic polypeptide, PP, Obinepitide, Pancreatic hormone, PH, Pancreatic icosapeptide, PI, PPY, PNP
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPY Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPY (HGNC:9327)
Synonyms PNP
Function [Pancreatic polypeptide]: Hormone secreted by pancreatic cells that acts as a regulator of pancreatic and gastrointestinal functions probably by signaling through the G protein-coupled receptor NPY4R2.
Cellular Location Secreted
Research Areas
Citations (0)
citation

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References

1.Am J Clin Nutr. 2011 Oct;94(4):967-72. 2.Pharm Res. 2012 Jun;29(6):1698-711.

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$ 385.00
Cat# AO1780a
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Availability: 7-10 days
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