|Application ||WB, IHC, FC, ICC, E|
|Reactivity||Human, Mouse, Rat, Monkey|
|Description||ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.|
|Immunogen||Purified recombinant fragment of human ACLY (AA: 306-502 ) expressed in E. Coli.|
|Formulation||Purified antibody in PBS with 0.05% sodium azide|
|Other Names||ATP-citrate synthase, 22.214.171.124, ATP-citrate (pro-S-)-lyase, ACL, Citrate cleavage enzyme, ACLY|
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ACLY Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.|
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1.J Biol Chem. 2010 Oct 15;285(42):32606-15. 2.Int J Cancer. 2010 May 15;126(10):2282-95.
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