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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NRCAM Antibody   

NRCAM Antibody

Purified Mouse Monoclonal Antibody

     
  • E - NRCAM Antibody AO1795a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - NRCAM Antibody AO1795a
    Figure 1: Western blot analysis using NRCAM mAb against human NRCAM recombinant protein. (Expected MW is 32.7 kDa)
  • WB - NRCAM Antibody AO1795a
    Figure 2: Western blot analysis using NRCAM mAb against HEK293 (1) and NRCAM (AA: 1192-1255)-hIgGFc transfected HEK293 (2) cell lysate.
  • IF - NRCAM Antibody AO1795a
    Figure 3: Immunofluorescence analysis of HepG2 cells using NRCAM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC, E
Primary Accession Q92823
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7D8C5
Isotype IgG1
Calculated MW 144kDa
Description Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. This gene is also expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms.
Immunogen Purified recombinant fragment of human NRCAM (AA: 1192-1255) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 4897
Other Names Neuronal cell adhesion molecule, Nr-CAM, Neuronal surface protein Bravo, hBravo, NgCAM-related cell adhesion molecule, Ng-CAM-related, NRCAM, KIAA0343
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/50
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNRCAM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NRCAM
Synonyms KIAA0343
Function Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons. Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier.
Cellular Location Cell membrane {ECO:0000250|UniProtKB:Q810U4}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q810U4}. Cell projection, axon {ECO:0000250|UniProtKB:Q810U4}. Secreted {ECO:0000250|UniProtKB:Q810U4}. Note=Detected at nodes of Ranvier {ECO:0000250|UniProtKB:Q810U4}
Tissue Location Detected in all the examined tissues. In the brain it was detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus
Research Areas
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Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. ;

References

1. Psychiatry Clin Neurosci. 2009 Feb;63(1):123-4. 2. Int J Neuropsychopharmacol. 2009 Feb;12(1):1-10.

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$ 385.00
Cat# AO1795a
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