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FGF4 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - FGF4 Antibody AO1799a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

    detail
  • WB - FGF4 Antibody AO1799a
    Figure 1: Western blot analysis using FGF4 mAb against human FGF4 recombinant protein. (Expected MW is 32.6 kDa)
    detail
  • WB - FGF4 Antibody AO1799a
    Figure 2: Western blot analysis using FGF4 mAb against HEK293 (1) and FGF4 (AA: 62-123)-hIgGFc transfected HEK293 (2) cell lysate.
    detail
  • FC - FGF4 Antibody AO1799a
    Figure 3: Flow cytometric analysis of NIH/3T3 cells using FGF4 mouse mAb (green) and negative control (red).
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  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
E
Primary Accession P08620
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 2D7D5
Isotype IgG1
Calculated MW 22kDa
Description The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its oncogenic transforming activity. This gene and FGF3, another oncogenic growth factor, are located closely on chromosome 11. Co-amplification of both genes was found in various kinds of human tumors. Studies on the mouse homolog suggested a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway.
Immunogen Purified recombinant fragment of human FGF4 (AA: 62-123) expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide.
Additional Information
Gene ID 2249
Other Names Fibroblast growth factor 4, FGF-4, Heparin secretory-transforming protein 1, HST, HST-1, HSTF-1, Heparin-binding growth factor 4, HBGF-4, Transforming protein KS3, FGF4, HST, HSTF1, KS3
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFGF4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FGF4 (HGNC:3682)
Function Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal limb and cardiac valve development during embryogenesis. May play a role in embryonic molar tooth bud development via inducing the expression of MSX1, MSX2 and MSX1-mediated expression of SDC1 in dental mesenchyme cells (By similarity).
Cellular Location Secreted.
Research Areas
Citations (0)
citation

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References

1.PLoS One. 2009;4(3):e4794. 2.Stem Cells. 2008 Mar;26(3):767-74.

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$ 385.00
Cat# AO1799a
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