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FGF4 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - FGF4 Antibody AO1800a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - FGF4 Antibody AO1800a
    Figure 1: Western blot analysis using FGF4 mAb against human FGF4 recombinant protein. (Expected MW is 32.6 kDa)
  • WB - FGF4 Antibody AO1800a
    Figure 2: Western blot analysis using FGF4 mAb against HEK293 (1) and FGF4 (AA: 62-123)-hIgGFc transfected HEK293 (2) cell lysate.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P08620
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 3A12G9
Isotype IgG1
Calculated MW 22kDa
Description The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its oncogenic transforming activity. This gene and FGF3, another oncogenic growth factor, are located closely on chromosome 11. Co-amplification of both genes was found in various kinds of human tumors. Studies on the mouse homolog suggested a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway.
Immunogen Purified recombinant fragment of humanFGF4 (AA: 62-123) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 2249
Other Names Fibroblast growth factor 4, FGF-4, Heparin secretory-transforming protein 1, HST, HST-1, HSTF-1, Heparin-binding growth factor 4, HBGF-4, Transforming protein KS3, FGF4, HST, HSTF1, KS3
Dilution E~~1/10000
WB~~1/500 - 1/2000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFGF4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FGF4
Synonyms HST, HSTF1, KS3
Function Plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation. Required for normal limb and cardiac valve development during embryogenesis.
Cellular Location Secreted.
Research Areas
Citations (0)

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Background

A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

References

1.PLoS One. 2009;4(3):e4794. 2.Stem Cells. 2008 Mar;26(3):767-74.

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$ 385.00
Cat# AO1800a
Size:
Quantity:
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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