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GSC Antibody

Purified Mouse Monoclonal Antibody

     
  • E - GSC Antibody AO1807a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - GSC Antibody AO1807a
    Figure 1: Western blot analysis using GSC mAb against human GSC recombinant protein. (Expected MW is 33.5 kDa)
  • WB - GSC Antibody AO1807a
    Figure 2: Western blot analysis using GSC mAb against HEK293 (1) and GSC (AA: 191-257)-hIgGFc transfected HEK293 (2) cell lysate.
  • FC - GSC Antibody AO1807a
    Figure 4: Flow cytometric analysis of Hela cells using GSC mouse mAb (green) and negative control (red).
  • IHC - GSC Antibody AO1807a
    Figure 5: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using GSC mouse mAb with DAB staining.
  • IHC - GSC Antibody AO1807a
    Figure 6: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using GSC mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession P56915
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 4C5D5
Isotype IgG1
Calculated MW 28.2kDa
Description This gene encodes a member of the bicoid subfamily of the paired (PRD) homeobox family of proteins. The encoded protein acts as a transcription factor and may be autoregulatory. A similar protein in mice plays a role in craniofacial and rib cage development during embryogenesis.
Immunogen Purified recombinant fragment of human GSC (AA: 191-257) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 145258
Other Names Homeobox protein goosecoid, GSC
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGSC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GSC
Function Regulates chordin (CHRD). May play a role in spatial programing within discrete embryonic fields or lineage compartments during organogenesis. In concert with NKX3-2, plays a role in defining the structural components of the middle ear; required for the development of the entire tympanic ring (By similarity). Probably involved in the regulatory networks that define neural crest cell fate specification and determine mesoderm cell lineages in mammals.
Cellular Location Nucleus.
Research Areas
Citations (0)

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Background

This gene encodes a member of the bicoid subfamily of the paired (PRD) homeobox family of proteins. The encoded protein acts as a transcription factor and may be autoregulatory. A similar protein in mice plays a role in craniofacial and rib cage development during embryogenesis. ;

References

1. Dev Biol. 2012 Feb 1;362(1):94-103. 2. Proc Natl Acad Sci U S A. 2006 Dec 12;103(50):18969-74.

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$ 385.00
Cat# AO1807a
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