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MCAM Antibody

Purified Mouse Monoclonal Antibody

     
  • E - MCAM Antibody AO1808a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - MCAM Antibody AO1808a
    Figure 1: Western blot analysis using MCAM mAb against human MCAM recombinant protein. (Expected MW is 37.7 kDa)
  • WB - MCAM Antibody AO1808a
    Figure 2: Western blot analysis using MCAM mAb against HEK293 (1) and MCAM (AA: 84-189)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - MCAM Antibody AO1808a
    Figure 3: Western blot analysis using MCAM mouse mAb against HUVE-12 (1), EVC-304 (2), HELA (3) and MCF-7 (4) cell lysate.
  • FC - MCAM Antibody AO1808a
    Figure 4: Flow cytometric analysis of MCF-7 cells using MCAM mouse mAb (green) and negative control (red).
  • IHC - MCAM Antibody AO1808a
    Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MCAM mouse mAb with DAB staining.
  • IHC - MCAM Antibody AO1808a
    Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using MCAM mouse mAb with DAB staining.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession P43121
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 6C3E6
Isotype IgG1
Calculated MW 71.6kDa
Description The protein encoded by this gene plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Immunogen Purified recombinant fragment of human MCAM (AA: 84-189) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 4162
Other Names Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, CD146, MCAM, MUC18
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMCAM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MCAM
Synonyms MUC18
Function Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis
Research Areas
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Background

The protein encoded by this gene plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. ; ;

References

1. Cancer Lett. 2013 Apr 28;330(2):150-62. 2. J Biol Chem. 2013 Jan 25;288(4):2571-9.

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$ 385.00
Cat# AO1808a
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