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ALCAM Antibody

Purified Mouse Monoclonal Antibody

     
  • E - ALCAM Antibody AO1815a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ALCAM Antibody AO1815a
    Figure 1: Western blot analysis using ALCAM mAb against human ALCAM recombinant protein. (Expected MW is 44.9 kDa)
  • WB - ALCAM Antibody AO1815a
    Figure 2: Western blot analysis using ALCAM mAb against HEK293 (1) and ALCAM (AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate.
  • IF - ALCAM Antibody AO1815a
    Figure 3: Immunofluorescence analysis of Hela cells using ALCAM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
  • FC - ALCAM Antibody AO1815a
    Figure 4: Flow cytometric analysis of JURKAT cells using ALCAM mouse mAb (green) and negative control (red).
  • IHC - ALCAM Antibody AO1815a
    Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ALCAM mouse mAb with DAB staining.
  • IHC - ALCAM Antibody AO1815a
    Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using ALCAM mouse mAb with DAB staining.
  • SPECIFICATION
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, ICC, E
Primary Accession Q13740
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 8E12C7
Isotype IgG2a
Calculated MW 65.1kDa
Description This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
Immunogen Purified recombinant fragment of human ALCAM (AA: 48-216) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 214
Other Names CD166 antigen, Activated leukocyte cell adhesion molecule, CD166, ALCAM, MEMD
Dilution E~~1/10000
WB~~1/500 - 1/2000
IF~~1/200 - 1/1000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALCAM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALCAM
Synonyms MEMD
Function Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines.
Research Areas
Citations (0)

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Background

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. ;

References

1. Vascul Pharmacol. 2011 Mar-Jun;54(3-6):93-9. 2. Int J Gynecol Cancer. 2011 Apr;21(3):523-8.

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$ 385.00
Cat# AO1815a
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