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NAPSA Antibody

Purified Mouse Monoclonal Antibody

     
  • E - NAPSA Antibody AO1818a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - NAPSA Antibody AO1818a
    Figure 1: Western blot analysis using NAPSA mAb against human NAPSA recombinant protein. (Expected MW is 40.9 kDa)
  • WB - NAPSA Antibody AO1818a
    Figure 2: Western blot analysis using NAPSA mAb against HEK293 (1) and NAPSA (AA: 20-158)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - NAPSA Antibody AO1818a
    Figure 3: Western blot analysis using NAPSA mouse mAb against rat liver tissue lysate.
  • IHC - NAPSA Antibody AO1818a
    Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NAPSA mouse mAb with DAB staining.
  • IHC - NAPSA Antibody AO1818a
    Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using NAPSA mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession O96009
Reactivity Human, Rat
Host Mouse
Clonality Monoclonal
Clone Names 10C4B8
Isotype IgG1
Calculated MW 45.4kDa
Description The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus.
Immunogen Purified recombinant fragment of human NAPSA (AA: 20-158) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 9476
Other Names Napsin-A, 3.4.23.-, Aspartyl protease 4, ASP4, Asp 4, Napsin-1, TA01/TA02, NAPSA, NAP1, NAPA
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNAPSA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NAPSA
Synonyms NAP1, NAPA
Function May be involved in processing of pneumocyte surfactant precursors.
Cellular Location Secreted.
Tissue Location Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes
Research Areas
Citations (0)

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Background

The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. ; ; ;

References

1. Lung Cancer. 2012 Jul;77(1):156-61. 2. Cancer Cytopathol. 2011 Oct 25;119(5):335-45.

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$ 385.00
Cat# AO1818a
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