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ABCG5 Antibody

Purified Mouse Monoclonal Antibody

     
  • E - ABCG5 Antibody AO1821a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - ABCG5 Antibody AO1821a
    Figure 1: Western blot analysis using ABCG5 mAb against human ABCG5 recombinant protein. (Expected MW is 32.7 kDa)
  • WB - ABCG5 Antibody AO1821a
    Figure 2: Western blot analysis using ABCG5 mAb against HEK293 (1) and ABCG5 (AA: 306-367)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - ABCG5 Antibody AO1821a
    Figure 3: Western blot analysis using ABCG5 mouse mAb against HL7702 (1), RAJI (2) and Jurkat (3) cell lysate.
  • FC - ABCG5 Antibody AO1821a
    Figure 4: Flow cytometric analysis of A549 cells using ABCG5 mouse mAb (green) and negative control (red).
  • IHC - ABCG5 Antibody AO1821a
    Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ABCG5 mouse mAb with DAB staining.
  • IHC - ABCG5 Antibody AO1821a
    Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ABCG5 mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, E
Primary Accession Q9H222
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 1B5E10
Isotype IgG1
Calculated MW 72.5kDa
Description The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia.
Immunogen Purified recombinant fragment of human ABCG5 (AA: 306-367) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 64240
Other Names ATP-binding cassette sub-family G member 5, Sterolin-1, ABCG5
Dilution E~~1/10000
WB~~1/500 - 1/2000
FC~~1/200 - 1/400
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsABCG5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ABCG5
Function Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile.
Cellular Location Membrane; Multi-pass membrane protein
Tissue Location Strongly expressed in the liver, lower levels in the small intestine and colon
Research Areas
Citations (0)

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Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. ; ;

References

1. PLoS One. 2012;7(5):e37972. 2. Biochemistry. 2010 Apr 27;49(16):3403-11.

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$ 385.00
Cat# AO1821a
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