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GPNMB Antibody

Purified Mouse Monoclonal Antibody

     
  • E - GPNMB Antibody AO1823a

    Black line: Control Antigen (100 ng);
    Purple line: Antigen(10ng);
    Blue line: Antigen (50 ng);
    Red line: Antigen (100 ng);

  • WB - GPNMB Antibody AO1823a
    Figure 1: Western blot analysis using GPNMB mAb against human GPNMB recombinant protein. (Expected MW is 47.0 kDa)
  • WB - GPNMB Antibody AO1823a
    Figure 2: Western blot analysis using GPNMB mAb against HEK293 (1) and GPNMB (AA: 31-260)-hIgGFc transfected HEK293 (2) cell lysate.
  • WB - GPNMB Antibody AO1823a
    Figure 3: Western blot analysis using GPNMB mouse mAb against PANC1 (1) and PC-3 (2) cell lysate.
  • IHC - GPNMB Antibody AO1823a
    Figure 4: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using GPNMB mouse mAb with DAB staining.
  • IHC - GPNMB Antibody AO1823a
    Figure 5: Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues using GPNMB mouse mAb with DAB staining.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, E
Primary Accession Q14956
Reactivity Human
Host Mouse
Clonality Monoclonal
Clone Names 7C10E5
Isotype IgG1
Calculated MW 64kDa
Description The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
Immunogen Purified recombinant fragment of human GPNMB (AA: 31-260) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Additional Information
Gene ID 10457
Other Names Transmembrane glycoprotein NMB, Transmembrane glycoprotein HGFIN, GPNMB, HGFIN, NMB
Dilution E~~1/10000
WB~~1/500 - 1/2000
IHC~~1/200 - 1/1000
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGPNMB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GPNMB
Synonyms HGFIN, NMB
Function Could be a melanogenic enzyme.
Cellular Location Cell membrane; Single-pass type I membrane protein. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Detected at the cell surface in different types of cancers cells, including glioblastoma multiforme cells and most melanoma cell lines
Tissue Location Up-regulated in various cancer cells, including in glioblastoma multiforme. Expressed in many melanoma cells, as well as in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level). Hardly detectable in healthy brain.
Research Areas
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Background

The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. ;

References

1. Prostate. 2012 Sep 15;72(13):1431-42. 2. Melanoma Res. 2010 Jun;20(3):184-90.

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$ 385.00
Cat# AO1823a
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